Application

Immunoblotting Standard (20 µl/lane)r>MMP Inhibition (assay dependent, see comments)

Biochem/physiol Actions

IC₅₀ against native human MMP-1 is ~1-5 nM.

Cell permeable: no

Reversible: no

Target IC₅₀: 1-5 nM against human MMP 1

Product does not compete with ATP.

Primary TargetHuman MMP 1

General description

Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a ~28 kDa protein by SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.

Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a 28 kDa protein under reducing conditions on SDS-PAGE.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm.171, 250.Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.Kodama, S. et al. 1987. Collagen Rel. Res.7, 341.Docherty, A.J.P. et al. 1985. Nature318, 66.Nagai, Y., et al. 1984. Jpn. J. Inflam.4, 123.

TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC₅₀ of 1-5 nM (see application references).

Packaging

3 µg in Plastic ampoule

Physical form

Lyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.

Reconstitution

Reconstitute with PBS to the desired concentration and mix gently until completely dissolved.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)